世界*實驗材料供應(yīng)商 Applied Genetics Laboratories, Inc   正式授權(quán)上海起發(fā)為其中國代理， appliedgenetics  在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 appliedgenetics  就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Hozier博士在佛羅里達州立大學分子生物物理研究所進行染色體復(fù)制研究生工作，并在Tübingen的馬克斯·普朗克研究所進行染色體分子組織研究工作，德國。他曾在明尼蘇達大學醫(yī)學院，佛羅里達州立大學佛羅里達州立大學（分子生物物理研究所）和新墨西哥州立大學醫(yī)學院任教。他于1984年創(chuàng)立了AGL。他的研究興趣集中在理解染色體畸變和染色體分子組織。他的臨床興趣包括使用DNA探針的高分辨率核型分析和熒光原位雜交，現(xiàn)在通常用于臨床工作來定義癌癥中的特定染色體重排和發(fā)育異常。他是美國醫(yī)學遺傳學院的研究員，并獲得美國醫(yī)學遺傳學委員會臨床細胞遺傳學認證。曾任多所大學和商業(yè)機構(gòu)的臨床實驗室主任。

Bryan K Hall，MS，CG（ASCP）^CM 技術(shù)總監(jiān) Bryan從佛羅里達理工學院獲得細胞和分子生物學碩士學位，對致突變性測定中使用的組織培養(yǎng)系進行染色體分析。他在人類，小鼠，大鼠，猴子等多種物種的細胞遺傳學分析和染色體鑒定方面擁有30年經(jīng)驗。布萊恩在約翰霍普金斯大學醫(yī)學院和密歇根大學兒科遺傳學系擔任過學術(shù)職務(wù)，并獲得了位于新墨西哥州阿爾伯克基市TriCore參考實驗室的臨床細胞遺傳學經(jīng)驗，負責細胞遺傳學實驗室的技術(shù)和監(jiān)管工作。除了染色體鑒定，Bryan精通染色體畸變分析，致突變性分析和熒光原位雜交（FISH），包括探針生產(chǎn)。他已經(jīng)在人類臨床組織，建立的細胞系，體細胞雜交體和實驗室小鼠的組織培養(yǎng)樣品上工作多年。他的分子生物學技術(shù)包括核酸純化，PCR，大插入克隆繁殖和操縱以及基因作圖。Bryan曾在AGL工作了8年，目前擔任技術(shù)總監(jiān)，他是Cell Line Characterization Studies的研究總監(jiān)。

Hybloc™ competitor DNA is the repetitive sequence fraction of genomic DNA. Hybloc™ competitor DNA may be effectively substituted for any other competitor DNA (i.e., COT-1 DNA®) for fluorescence in situ hybridization (FISH), Southern blots, microarray technology, or any other protocol requiring competition to prevent repetitive DNA from binding to the target. Hybridization with Hybloc™ competitor DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA. Hybloc™ competitor DNA is available for the following species: Mouse, Rat, Human, Bovine, Ovine, Porcine, Chicken, and Canine.

Product: Ovine Hybloc DNA

Catalog #: OHB

Concentration: 1 mg/ml (A260)

Source: Male Ovine Tissues

Storage: -20°C

Description:

Ovine Hybloc DNA is the repetitive sequence fraction of genomic DNA obtained from male ovine tissues by extracting, shearing, denaturing, and reannealing DNA under conditions that enrich for repetitive sequences.

Ovine Hybloc DNA may be effectively substituted for any other competitor DNA (e.g., COT-1 DNA , Salmon Sperm DNA, Genomic DNA, etc.) to suppress cross-hybridization to ovine repetitive sequences for in situ hybridization, Southern blots, microarray technology, or any other protocol requiring competition to block repetitive DNA from binding to the target. Hybridization with Hybloc DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA.

How Supplied:

Ovine Hybloc DNAs are supplied in various quantities at 1mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA.

Quality Control Assay:

Determination of concentration from the A260 and determination of the A260/A280 ratio (1.8 to 2.0); determination of molecular size: >80% of Ovine Hybloc DNA will run as a broad band between the 50 bp and 600 bp size range.

References:

Landegent JE, Jansen in de Wal N, Dirks RW, Baao F, van der Ploeg M. Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Human Genetics 1987 Dec;77(4):366-70.

Lengauer C, Riethman H, Cremer T. Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers. Human Genetics 1990 Nov;86(1):1-6.

Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988 Nov;80(3):224-34.

Lichter P, Tang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward DC. High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 1990 Jan5;247(4938):64-9.

Scalzi JM, & Hozier, JC. Comparative genome mapping: mouse and rat homologies revealed by fluorescence in situ hybridization. Genomics 1998 Jan1;47(1):44-51.

COT-1 DNA is a registered trademark of Invitrogen Corporation.

部分產(chǎn)品報價如下：

我們公司zui大優(yōu)勢是強大的采購，

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6：我們還是Santa,Advanced Biotechnologies Inc, IBEX Technologies Inc.,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. Dako,Inc, GePromegaridege,IBEX Technologies Inc.  Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,IBEX Technologies Inc. , Polysciences,prospecbi, QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。
7：我們還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發(fā)，歡迎合作。