seracare 5120-0047說(shuō)明書(shū)

細(xì)節(jié)：

Most sensitive chromogenic substrate for peroxidase detection, more sensitive than ABTS and OPD. It develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm. Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA. TMB can also be used in blotting applications with the purchase of the KPL TMB Membrane Enhancer.

Two-component format of 3, 3', 5, 5' - tetramethylbenzidine, simply mix and use. Has longer shelf life than one-component TMB substrates.

用于過(guò)氧化物酶檢測(cè)的靈敏的顯色底物，比ABTS和OPD更靈敏。在過(guò)氧化物酶標(biāo)記的偶聯(lián)物存在下，它會(huì)產(chǎn)生深藍(lán)色，可在 650 nm 處測(cè)量。當(dāng)酸化停止并在 450 nm 處讀取時(shí)變黃。推薦用于 ELISA。購(gòu)買(mǎi) KPL TMB 膜增強(qiáng)劑后，TMB 也可用于印跡應(yīng)用。

3、3'、5、5'-四甲基聯(lián)苯胺雙組份形式，混合使用簡(jiǎn)單。比單組分TMB基材具有更長(zhǎng)的保質(zhì)期。

Reference #: 50-76-00